Oxford Nanopore Technologies Long Read Sequencing Live Workshop: Single Cell RNA-Seq Analysis
To Know
About this Class
Are you looking to expand the reach of your sequencing to enable what long read technologies can provide? Please join Oxford Nanopore Technologies (ONT) bioinformatics specialists on a deep dive into getting the most from your long read sequencing data.
This workshop will cover the details of analyzing single-cell RNA sequencing data using our EPI2ME pipeline wf-single-cell. This workflow provides access to industry standard tools for primary processing of single-cell data including deconvolution, quality control, gene and transcript identification, and data visualization. Participants will learn about the EPI2ME software, pipeline details, and work with an ONT bioinformatics expert in a hands-on data analysis training exercise.
This in-person, hands-on workshop gives learners the opportunity to learn the Nanopore Single Cell RNA-Seq Analysis Software Workflow. Taught by Nanopore personnel, with assistance from the CCR Genomics Core and BTEP, this session will run in the morning. Space is limited. If your plans change and you cannot attend, please cancel your registration. There will be no hybrid option for this class, it is in-person only.
Agenda:
9:00-9:15 – Check in & distribute materials
9:15-9:30 – Data analysis intro from Oxford Nanopore Technologies (MinKNOW/EPI2ME/other advanced tools)
9:30-10:00 – Introduction to Single Cell RNA-seq data analysis with Oxford Nanopore Technologies
10:00-11:15 – Hands on data analysis: Single Cell EPI2ME App, nextflow command line, and Biowulf HPC demo
11:15-12:00 – Downstream analysis with Seurat
12:00-12:30 – Closing and Q&A