Single Cell RNA-Seq Data Analysis in Partek Flow

About this Class

Registration is required to join this event. If you have not registered, please do so now. Register Speaker: Uchenna Emechebe, Ph.D., Field Application Scientist, Partek Incorporated. Partek® Flow® bioinformatics software which is available to NCI researchers provides a singular environment that enables the analysis and visualization of NGS data with no programming or command line expertise. Join us for these two online sessions where a Partek® scientist will demonstrate how to pre-process, analyze, and visualize Bulk RNA-Seq and/or Single Cell RNA-Seq data using Partek Flow’s point and click features. During this session, attendees will learn how to perform QA/QC, custom filtration of single cell datasets, normalization, clustering and classification, biological interpretation and visualization of results using UMAP, t-SNE, volcano plots. In addition, attendees of the webinar will: • Become familiar with various single cell normalization and batch correction methods. • Learn how to summarize gene expression values across different subsets of cells for any given set of genes and visualize the results using a bubble plot or a heat map.