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Library ChIP preparation: What happens if there are too many PCR cycles?

What happens if there are too many PCR cycles?

1

1 Answer:

You get a lot of duplicated reads downstream in the analysis which will decrease the amount of information you get from your ChIP-seq. You should be able to detect this in the lab prior to sequencing as a random super sharp band on your gel, Bioanalyzer, or TapeStation. - answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.

Answered on June 5th, 2020 by