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Library/ChIP preparation: Is it okay to use frozen samples or is it better to use fresh cells?

Is it okay to use frozen samples or is it better to use fresh cells?

1

1 Answer:

There should be no appreciable difference between using frozen vs fresh cells. That being said, since the ChIP method uses fixation to stabilize the DNA-protein interactions, it is advisable to first fix the cells for long term storage. There is the possibility that freeze thaw of frozen tissues or cells may alter interactions. - answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.

Answered on June 5th, 2020 by