Are you new to using Partek® Flow® bioinformatic software or need to brush up on your skills? Then join this online training series where a Partek expert will teach you how to use Partek Flow and go over best practices. Join each session or just those of interest.
To register for any or all sessions: Registration Link
Tuesday, March 30, 1 PM – Partek Flow Basic Components
- How to transfer files to Flow server
- Import data to a project (fastq, bam, bcl, .txt, .h5, seurat object)
- Sample attribute management
- Project management (task log, sharing, task details, project and data export, and importing and re-using pipelines)
- Library file management
- Gene list management
Thursday, April 1, 1 PM – Bulk RNA-Seq Data Analysis
- QA/QC
- Trimming bases and filtering reads
- Alignment
- Quantification
- Filtering features and normalization
- Differential analysis
- Alternative splicing detection
- Biological interpretation
- Data visualization: PCA scatterplot, volcano plot, heatmap, dot plot, chromosome view etc.
Tuesday, April 6, 1 PM – Single Cell RNA-Seq Data Analysis: Basic Functions for a Single Sample
- Single cell QA/QC (QC cells and filter, QC features and filter)
- Normalization methods
- Dimension reduction methods (PCA, tSNE, UMAP)
- Clustering analysis (Graph-based clustering, K-means clustering)
- Cell type classification in Data Viewer
- Visualization: scatterplot, violin plot
- Trajectory analysis
Thursday, April 8, 1 PM – Single Cell RNA-Seq Data Analysis: Advanced Functions for Multiple Samples
- Batch removal methods
- Differential analysis
- Sample composition analysis
- Pseudo bulk analysis
- Visualizartion: dot plot, box plot, violin plot, volcano plot, heatmap, bubble map, bar chart, pie chart
- Spatial transcriptome
- CITE-Seq data analysis
To set up your Partek Flow account please see: https://partekflow.cit.nih.gov/