Bioinformatics Training and Education Program

BTEP: Introduction to RNA-Seq, Monday Nov. 5, 2018 with Peter Fitzgerald, Ph.D.

Nov. 5, 2018

9:30 – 11:30 AM

NIH Bldg 10, FAES classroom 3, B1C207

Presenter: Peter Fitzgerald, Ph.D.,  CCR Genome Analysis Unit

This introductory lecture will provide an overview of RNA-Seq technology, its various application and shortcomings, as well as detailing the steps involved in analyzing the resulting data.

The specific topics covered include:

  1. What is RNA-Seq?
  2. What can it be used for?
  3. Sequencing platforms
  4. Quality Control steps
  5. Experimental Design
  6. Data Analysis Workflows
  7. Identifying differentially expressed gene
  8. Discussion of relevant file formats and data conversion tools
  9. Approaches to detect splice variants and fusion genes
  10. NCI specific tools and software