About this Class

Please note class will start at 10 AM and end at 12 noon. Thank you! Attention: This class is limited to 28 people due to the size of the room. Please add your name to the waiting list if you would like another training scheduled. Hands On Training on Bulk RNA-Seq Data Analysis in Partek Flow Please bring your own computer for this hands-on training. If you need to borrow a computer please email ncibtep@nih.gov Abstract: Students will learn how to use the point-and-click interface in Partek Flow to go from raw data to experimental results for RNA-Seq analysis: - Import data from .fastq files - Perform QA/QC (Pre-alignment QA/QC, Post-alignment QA/QC) - Trim bases - Align reads to reference genome - Quantify gene/transcript abundance - Normalize gene counts - Detect differentially expressed genes - Filter a gene list - Identify enriched KEGG pathway and/or GO terms - Visualization:

• • • Heat maps
    • Volcano plots
    • PCA scatterplot
    • Dot plots
    • Hierarchical clustering
    • Chromosome view
    • And more

If you are unable to attend in person, WebEx will be provided. Meeting link: https://cbiit.webex.com/cbiit/j.php?MTID=m4ddd63165233af6473f4ce950642aecc Meeting number: 738 100 303 Password: AcCxyy$5 Host key: 544130 More ways to join Join by video system Dial 738100303@cbiit.webex.com You can also dial 173.243.2.68 and enter your meeting number. Join by phone 1-650-479-3207 Call-in number (US/Canada) Access code: 738 100 303

Files

• PartekFlowRNA-SeqTrainingHandout_November2018-2.pdf: |
• PartekFlowNewFeatureList_AUG_2019-1.pdf: |