BTEP: Microarray and Methylation Data Analysis in Partek Genomics Suite with Pathway

When: Aug. 28th, 2019 9:00 am - 11:00 am

This class has ended.

Video Archive *Class recordings may take ~48 hours to become available.

To Know

Where: Building 37, Room 4041/4107
Organized By: BTEP
Presented By: Xiaowen Wang (Partek)

About this Class

Please bring your computer with Partek Genomics Suite to this hands-on workshop. You'll need to submit a request through “NCI at Your Service” to obtain the access to this software. Partek will demonstrate both Microarray and Methylation Data Analysis in Partek Genomics Suite with Pathway ***Microarray Data Analysis in Partek Genomics Suite with Pathway*** Abstract: The class will start with an overview of Partek Genomics Suite with Pathway and followed by a hands-on tutorial session using a sample Gene Expression dataset. The hands-on session will help attendees gain expertise on the following topics: data import, QA/QC, exploratory data analysis, differential expression analysis, visualizations, and biological interpretation as well as other basic analysis in Partek Genomics Suite with Pathway.

Data Import
QA/QC
Exploratory Data Analysis
Differential Expression Analysis
Visualization (PCA, hierarchical clustering, dot plot, chromosome view, volcano plot, etc.)
Biological Interpretation

***Microarray Methylation Data Analysis in Partek Genomics Suite*** The class will use the Illumina 450K Methylation array data. The workflow can also be used on HumanMethylation27 (27K) and HumanMethylationEPIC (850K) BeadChips. Attendees will learn how to perform QA/QC, detect differential methylation, find genes overlapping CpG loci and biological interpretation in Partek Genomics Suite with Pathway. - Import data from Illumina methylation array in .idat files - Methylation array-specific normalization - Perform QA/AC - Detection of differentially methylated CpG loci - Creating list of loci of interest