ChIP Seq Data Analysis Using Galaxy
To Know
About this Class
You will learn how to analyze ChIP-Seq data using Galaxy, a user-friendly web-based platform that makes bioinformatics accessible to researchers with little or no programming experience.
To get you started, Drs. Chunhua Yan and Qingrong Chen will:
• Introduce the fundamentals of ChIP-Seq data analysis.
• Demonstrate commonly used ChIP-Seq analysis tools and workflows in Galaxy.
• Prepare you to run a basic ChIP-Seq analysis workflow in Galaxy for transcription factor binding site identification.
During the self-paced hands-on exercises, you will analyze Illumina ChIP-Seq data in Galaxy and learn how to:
• Perform quality control on raw ChIP-Seq data.
• Map sequencing reads to a reference genome.
• Generate alignment statistics and assess mapping quality.
• Identify enriched binding regions, or peak calling, using Model-based Analysis of ChIP-Seq (MACS).
• Annotate identified peaks.
• Visualize enriched genomic regions.
Whether you're new to bioinformatics or looking to strengthen your ChIP-Seq data analysis skills, this workshop will give you practical experience and confidence to perform your own analyses—no programming required.