Hands-on: RNA-Seq Data Analysis with Partek Genomics Suite

About this Class

This training session will focus on Gene Expression analysis and the rich set of results possible from RNA-Seq based studies. In addition to differential gene expression, researchers also have the opportunity to discover SNPs, and important alternative splicing patterns that result in allele-specific expression. The following topics will be discussed:

• Alignment
• Pre/Post Alignment QA/QC 
• Trimming, filtering reads
• Quantification
• Differential Expression Detection using Gene Specific Analysis
• Variants/Indel Detection and Annotation
• Visualization (PCA, Dot Plot, Chromosome View, etc.)
• Allele Specific Expression Analysis
• Alt-splicing Detection
• Biological interpretation: Gene Set Analysis and Pathway Analysis

Partek Genomics Suite is  available to all researchers affiliated with CCR 

Course Materials:

• Introduction to NGS (PDF)