BTEP: Introduction to Gene Expression Microarray Data Analysis

Introduction to Gene Expression Microarray Data Analysis

When: Oct. 2nd, 2012 2:00 pm - 3:30 pm

Where:

Building 37, Room 4041/4107

Organizer:

BTEP

This class has ended.

Video Archive *Class recordings may take ~48 hours to become available.

Microarray Technology and Preprocessing
- Quality Control
- Normalization Using MAS5 and RMA
- Filtering
- Batch Effect Correction
Basic Statistical Tests for Differentially Expressed Genes
- T-test
- ANOVA
- SAM
- Calculating False Discovery Rate
- Principal Components Analysis and Clustering
Functional and Network Analysis
1. Pathway Analysis
  - Ingenuity Pathway Analysis (IPA)
  - Gene Set Enrichment Analysis (GSEA)
  - Fishers Exact Test
2. Motif Enrichment Analysis
  - IPA motif enrichment analysis for transcription factor and miRNA motifs
  - PSCAN for transcription factor motifs
  - Fishers Exact Test for transcription factor and miRNA motifs
3. Network Reconstruction
  - ARACNE
  - Multivariate Regression