ncibtep@nih.gov

Bioinformatics Training and Education Program

Introduction to Gene Expression Microarray Data Analysis

Introduction to Gene Expression Microarray Data Analysis

 When: Oct. 2nd, 2012 2:00 pm - 3:30 pm

This class has ended.
Video Archive *Class recordings may take ~48 hours to become available.
To Know
  • Where: Building 37, Room 4041/4107
  • Organized By: BTEP

About this Class

  1. Microarray Technology and Preprocessing
    • Quality Control
    • Normalization Using MAS5 and RMA
    • Filtering
    • Batch Effect Correction
  2. Basic Statistical Tests for Differentially Expressed Genes
    • T-test
    • ANOVA
    • SAM
    • Calculating False Discovery Rate
    • Principal Components Analysis and Clustering
  3. Functional and Network Analysis
    1. Pathway Analysis
      • Ingenuity Pathway Analysis (IPA)
      • Gene Set Enrichment Analysis (GSEA)
      • Fishers Exact Test
    2. Motif Enrichment Analysis
      • IPA motif enrichment analysis for transcription factor and miRNA motifs
      • PSCAN for transcription factor motifs
      • Fishers Exact Test for transcription factor and miRNA motifs
    3. Network Reconstruction
      • ARACNE
      • Multivariate Regression
         

Course Materials: Lecture Slides in PDF Format