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Should we always use histone markers just to see if our sequencing is working for our samples?
Should we always use histone markers just to see if our sequencing is working for our samples?
1 Answer:
No, there is no reason to start with a histone marker just to see if sequencing is working for your samples. If you are worried about a specific cell type for any reason or this is your first time making a ChIP-seq library, it is beneficial to do a positive control experiment but that does not need to be a histone marker. In fact, an IP of a generic histone like H3 can actually be difficult to differentiate from an input sometimes and may make it difficult for you to feel confident in the results. - answered by Tovah Markowitz, Paul Schaughency, Vishal Koparde.
Answered on June 5th, 2020 by amy.stonelake@nih.gov