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Spike-ins: At what stage of the ChIP experiment do you introduce the spike-ins?
At what stage of the ChIP experiment do you introduce the spike-ins?
1 Answer:
There are a few different stages when you can add the spike-ins. The most important thing is to make sure they are combined prior to the pull-down step. This includes combining cells together, combining spheroplasts together, combining nuclei together, or combining fragmented DNA together. by Tovah Markowitz, Paul Schaughency, Vishal Koparde.
Answered on June 5th, 2020 by amy.stonelake@nih.gov